AEO Answer · Zoos & Aquariums

How Should a Zoo Quarantine Facility Be Designed Safely?

By Jeremy Mills, CEO & Founder, Apex Grid Engineering — USAF Veteran. · Updated 2026-09-15

Zoo quarantine facilities isolate new arrivals with dedicated HVAC and no shared air, separate entries with disinfection protocols, one-way staff and equipment workflows, and drainage isolated from exhibit systems — designed assuming every incoming animal carries a pathogen, every time.

I'm Jeremy Mills, CEO & Founder of Apex Grid Engineering and a U.S. Air Force veteran. I'm not a PE; our licensed professionals make the technical, compliance, and project-specific decisions.

Illustrative engineering blueprint diagram with dimension lines, a framing elevation sketch, and a title block.

Isolation as an engineered system

Quarantine isolation works when every pathway a pathogen could travel is deliberately broken. Air gets its own system with no recirculation. Water and drainage stay separate from exhibit systems. People enter through dedicated vestibules with clothing changes and disinfection. Equipment lives in the building and never leaves without sterilization. I map each pathway — air, water, people, equipment, waste, vectors — and design a break into every one, because pathogens exploit whichever pathway the design forgot. Pressure relationships do quiet work in quarantine. Holding air at negative pressure relative to corridors means air flows into animal spaces, not out of them, carrying any airborne concern inward rather than toward staff and the rest of the facility. I verify these relationships with measurements during commissioning, not just design intent, because a door left open or a fan running backward can reverse the whole scheme.

Workflows that protect the collection

The building has to make biosecurity the easy choice. I lay out quarantine so staff enter through one point, suit up, work from the healthiest animals toward the sickest, and exit through disinfection — a one-way flow with no reason to backtrack. Dedicated storage keeps quarantine tools from migrating to other buildings. Sight lines let supervisors see compliance without hovering. When the workflow is designed in, staff follow it because it's simply how the building works. Animal welfare during quarantine gets equal attention. Weeks of isolation in a barren box produces stressed animals and unreliable health data, so I design quarantine holding with enrichment, natural light, proper climate, and space to move. Veterinary access — squeeze mechanisms, shift doors, exam areas — is built in so health screening doesn't require stressful captures. A quarantine building that cares for animals gets better diagnostic results and healthier introductions.

What to get right before construction

Quarantine projects fail when the building is designed as generic holding with an isolation label. True quarantine needs its own HVAC, its own entries, its own drainage, and its own equipment from the first sketch — retrofitting isolation into a shared building never fully works. I also coordinate with the veterinary team on the species the facility will actually quarantine, because a building sized for antelope won't serve giraffes. Commissioning proves the isolation: airflow measurements in every space, pressure relationship verification with doors operating normally, drainage cross-connection checks, and full workflow walkthroughs with the staff who'll run the building. I also test the failure modes — a fan failure, a door propped open — to confirm the isolation degrades safely rather than silently. Quarantine that passes these tests actually protects the collection. Here's the checklist I hand owners.

  • Give quarantine fully dedicated HVAC with exhaust-only air and no recirculation anywhere
  • Design one-way staff flow: single entry, suiting vestibule, work clean-to-dirty, disinfected exit
  • Break every pathogen pathway: separate air, water, drainage, equipment, waste, and entry points
  • Build welfare into isolation: enrichment, natural light, climate, and low-stress veterinary access
  • Commission the isolation itself: pressures, airflows, and workflows verified before first arrival

What else do project teams ask?

How long do new zoo animals stay in quarantine?
It varies by species and regulation, but thirty days is a common minimum, with some species and situations requiring longer. The period has to cover the incubation of the diseases being screened for, plus time for test results. I design quarantine housing for extended stays — enrichment, proper climate, and space — because a stressed animal in a bare box doesn't give reliable health readings.
Why can't quarantine share air with other zoo buildings?
Airborne pathogens travel on shared air the way smoke travels through ductwork. A quarantine ward on the same air handler as the primate house is quarantine in name only. I give quarantine its own HVAC with dedicated exhaust and no recirculation, so the air in the building has exactly one path: in through the intake, out through the exhaust, never into the collection.
What happens inside a zoo quarantine facility?
Health screening: veterinary exams, blood work, fecal tests, vaccinations, and observation for signs of illness — all while the animal is handled with dedicated equipment by staff following isolation protocols. It's also acclimation time: diet transitions, behavioral assessment, and training for the medical behaviors the new home will need. The building has to support weeks of this work comfortably.
How is quarantine waste handled safely at zoos?
Bedding, waste, and washdown water are treated as potentially infectious: bagged and disposed through medical or infectious waste streams, with drainage to sanitary and no cross-connections. I design the waste path to be short and dedicated — the less distance infectious material travels through the building, the fewer surfaces it can contaminate.

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